In this study, teachers’ profiles in terms of their instructional and professional practices were defined by using multilevel latent profile analysis. For this study, Turkish national data from TALIS 2018 (N = 6861 teachers from 443 schools) were utilized. Five teacher profiles that differed especially in terms of “clarity of instruction” emerged. Members of Profile E are the teachers using instructional practices most, and the members of Profile A are the ones using these practices least frequently. Results indicated the important role of both the “school climate for innovativeness” and school location as contextual factors in predicting profile memberships.