This study developed and validated the Science Practice Scale (SPS) and investigated the implementation of scientific teaching practices by Grade 8 science teachers in Jordan. The responses of 235 8th grade science teachers who participated in the Trends in International Mathematics and Science Study [TIMSS] of 2019 were analysed using the Rasch measurement. We found significant evidence supporting the construct validity of the SPS scale. The results showed that the estimated difficulty level of implementing the practices varies from -1.26 logit (observing natural phenomena) to 2.27 logit (conducting fieldwork), indicating that the level of conducting field experiments was very low. The results of the t-test (t = 0.267, df = 222, p<0.05) indicated that no statistically significant differences existed between male and female science teachers in implementing scientific practices. The Ministry of Education of Jordan is encouraged to organise specific professional development programmes to promote science teachers’ implementation of these practices, especially with regards to organising fieldwork and giving their pupils opportunities to design and conduct experiments and present data. The current study provides recommendations for improving the TIMSS teacher questionnaire and introduces suggestions for further research.